Alkaline transition of phytocyanins: a comparison of stellacyanin and umecyanin.

Crystal structures of oxidized and reduced stellacyanin from horseradish roots.

Umecyanin (UMC) is a type 1 copper-containing protein which originates from horseradish roots and belongs to the stellacyanin subclass of the phytocyanins, a ubiquitous family of plant cupredoxins. The crystal structures of Cu(II) and Cu(I) UMC have been determined at 1.9 and 1.8 A, respectively. The protein has an overall fold similar to those of other phytocyanins. At the active site the cupr...

Preparation and spectroscopic studies of cobalt(II)-stellacyanin.

The cobalt(II) derivative of the "blue" copper protein stellacyanin has been prepared, and its visible-ultraviolet spectrum is reported. Tryptophan fluorescence quenching and p-mercuribenzoate titration results strongly suggest that Co(II) and Cu(II) compete for the same stellacyanin binding site and that a cysteine sulfur atom is coordinated in both cases. This interpretation is supported by t...

Transition from Paleogene Normal Calc-Alkaline to Neogene Adakitic-Like Plutonism and

Structural properties of stellacyanin, a copper mucoprotein from Rhus vernicifera, the Japanese lac tree.

Stellacyanin, or Rhus vernicijera blue protein, was purified from lac acetone powders. It is 20% carbohydrate and 20% hexosamine with the remainder being a polypeptide matrix of 108 amino acid residues in which is embedded a single cupric atom. The molecule contains no methionine or valine. Optical rotatory dispersion and optical and electron paramagnetic resonance (EPR) spectral studies show t...

Synthesis of Zeolites, Merlinoite Minerals of Damavand’s Natural Clinoptiloite Zeolites Mineral in Hydrothermal Conditions, under the Influence of Alkaline

In the present research synthesis of zeolites, merlinoite minerals of natural clinoptiloite mineral in the presence of alkaline mediums, was investigated. The analyzed variants are a result of a concentration of Na+ and K+ ions, temperature and time. Phase transition of clinoptiloite was conducted in the presence of an 8 molar potash solution (KOH) and a 4 molar soda solution (NaOH). Results in...